Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (23): 3710-3715.doi: 10.3969/j.issn.2095-4344.1318

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Susceptibility of ankylosing spondylitis early-onset hip ankylosis and human leukocyte antigen-B27 subtypes 

Xiong Chunxiang1, 2, Wei Xiaochun3, Yin Dong2, Huang Yu2, Du Chang2, Mo Bingfeng2   

  • Received:2019-03-07 Online:2019-08-18 Published:2019-08-18
  • Contact: Yin Dong, MD, Master’s supervisor, Postdoctoral coordinator, Chief physician, Department of Orthopedics, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Xiong Chunxiang, MD, Attending physician, Postdoctoral Research Station of the People’s Hospital of Guangxi Zhuang Autonomous Region and Postdoctoral Research Station of Clinical Medicine of Shanxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Department of Orthopedics, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

     the Major Research and Development Project of Guangxi Zhuang Autonomous Region, No. AB16380230 (to YD); the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2015GXNSFAA139200 (to YD); the Medical and Health Self-Financing Project of Guangxi Zhuang Autonomous Region, No. Z2016609 (to XCX)

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Abstract:

BACKGROUND: Preliminary study has shown that positive for human leukocyte antigen-B27 is closely related to ankylosing spondylitis early-onset hip ankylosis.
OBJECTIVE: To study the susceptibility of ankylosing spondylitis early-onset hip ankylosis and human leukocyte antigen-B27 gene subtype, and to explore the role of human leukocyte antigen-B27 gene subtype in the pathogenesis of ankylosing spondylitis early-onset hip ankylosis.
METHODS: Three hundred cases of ankylosing spondylitis with early-onset hip ankylosis and 300 cases of ankylosing spondylitis without early-onset hip ankylosis were collected. The study was approved by the Ethics Committee of the People’s Hospital of Guangxi Zhuang Autonomous Region, and all the participants and their guardians signed the informed consents. A case-control study was conducted, and the cases of ankylosing spondylitis with early-onset hip ankylosis were confirmed by radiological examinations (hip MRI, CT, pelvic X-ray film) within 5 years. The general condition and clinical manifestation of the subjects were collected. The 2 mL venous blood of each subject was collected for detecting the 29 human leukocyte antigen-B27 genotypes by PCR-SSP high resolution technique. The constituent ratio of human leukocyte antigen-B27 gene subtypes was calculated and relative risk was analyzed.
RESULTS AND CONCLUSION: (1) The total positive rate of human leukocyte antigen-B27 detection in the two groups was 93.67% (562 cases), 99.33% in the ankylosing spondylitis with early-onset hip ankylosis group, and 88.59% in ankylosing spondylitis without early-onset hip ankylosis. (2) Six of the 29 types of human leukocyte antigen-B27 subtypes were found, which were B*2702, B*2703, B*2704, B*2705, B*2706 and B*2713. The two groups were mainly B*2704 and B*2705 subtypes. The proportion of B*2704 subtypes in the ankylosing spondylitis without early-onset hip ankylosis group was highest (P < 0.01). The highest proportion in the ankylosing spondylitis with early-onset hip ankylosis group was B*2705 (P < 0.01). (3) B*2705 subtype was associated with the risk of ankylosing spondylitis early-onset hip ankylosis (OR=1.896, 95%CI: 1.221-3.218). (4) In summary, the main subtypes of ankylosing spondylitis patients are B*2704 and B*2705, and the B*2705 subtype is a susceptible gene that causes early-onset hip ankylosis in ankylosing spondylitis patients. The subtype of B*2705 can be used as an important reference index for early diagnosis of ankylosing spondylitis early-onset hip ankylosis.

Key words: ankylosing spondylitis, hip ankylosis, human leukocyte antigen, early-onset hip ankylosis, HLA-B27

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